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Solute carrier family 25 member 33 (SLC25A33) regulates mitochondrial ROS (mtROS) and mitochondrial DNA (mtDNA) production in response to platelet-derived growth factor (PDGF), thereby promoting mtDNA release into the cytosol. (A) Representative immunofluorescence images of MitoSOX Red (left panel; scale bar=10 μm) and quantification of fluorescence intensity (right panel) in PDGF-treated vascular smooth muscle cells (VSMCs) with or without SLC25A33 -targeting small interfering RNA (siRNA). (B) Effect of SLC25A33 knockdown on mitochondrial and cytosolic uridine 5’-triphosphate (UTP) levels in PDGF-treated VSMCs. (C) Effect of SLC25A33 knockdown on total mtDNA levels in PDGF-treated VSMCs. (D) Representative immunofluorescence images <t>acquired</t> <t>using</t> <t>6-O-propynyl-dG</t> (PdG) and click chemistry (left panel; scale bar=5 μm) and quantification of PdG (right panel) in PDGF-treated VSMCs with or without SLC25A33 -targeting siRNA. (E) Representative immunofluorescence images of double-stranded DNA (dsDNA) and mitochondria (left panel; scale bar=10 μm) and quantification of cytosolic dsDNA foci (right panel) in PDGF-treated VSMCs with or without SLC25A33 -targeting siRNA. si SLC25A33 is referred to as siA33. Data in the bar graphs represent the mean±standard error of the mean of three independent measurements. Ctrl, control; NS, not significant; DAPI, 4’,6-diamidino-2-phenylindole. a P <0.05, b P <0.01, c P <0.001.
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Solute carrier family 25 member 33 (SLC25A33) regulates mitochondrial ROS (mtROS) and mitochondrial DNA (mtDNA) production in response to platelet-derived growth factor (PDGF), thereby promoting mtDNA release into the cytosol. (A) Representative immunofluorescence images of MitoSOX Red (left panel; scale bar=10 μm) and quantification of fluorescence intensity (right panel) in PDGF-treated vascular smooth muscle cells (VSMCs) with or without SLC25A33 -targeting small interfering RNA (siRNA). (B) Effect of SLC25A33 knockdown on mitochondrial and cytosolic uridine 5’-triphosphate (UTP) levels in PDGF-treated VSMCs. (C) Effect of SLC25A33 knockdown on total mtDNA levels in PDGF-treated VSMCs. (D) Representative immunofluorescence images <t>acquired</t> <t>using</t> <t>6-O-propynyl-dG</t> (PdG) and click chemistry (left panel; scale bar=5 μm) and quantification of PdG (right panel) in PDGF-treated VSMCs with or without SLC25A33 -targeting siRNA. (E) Representative immunofluorescence images of double-stranded DNA (dsDNA) and mitochondria (left panel; scale bar=10 μm) and quantification of cytosolic dsDNA foci (right panel) in PDGF-treated VSMCs with or without SLC25A33 -targeting siRNA. si SLC25A33 is referred to as siA33. Data in the bar graphs represent the mean±standard error of the mean of three independent measurements. Ctrl, control; NS, not significant; DAPI, 4’,6-diamidino-2-phenylindole. a P <0.05, b P <0.01, c P <0.001.
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cuaac cell reaction buffer kit bttaa based - by Bioz Stars, 2026-10
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Solute carrier family 25 member 33 (SLC25A33) regulates mitochondrial ROS (mtROS) and mitochondrial DNA (mtDNA) production in response to platelet-derived growth factor (PDGF), thereby promoting mtDNA release into the cytosol. (A) Representative immunofluorescence images of MitoSOX Red (left panel; scale bar=10 μm) and quantification of fluorescence intensity (right panel) in PDGF-treated vascular smooth muscle cells (VSMCs) with or without SLC25A33 -targeting small interfering RNA (siRNA). (B) Effect of SLC25A33 knockdown on mitochondrial and cytosolic uridine 5’-triphosphate (UTP) levels in PDGF-treated VSMCs. (C) Effect of SLC25A33 knockdown on total mtDNA levels in PDGF-treated VSMCs. (D) Representative immunofluorescence images <t>acquired</t> <t>using</t> <t>6-O-propynyl-dG</t> (PdG) and click chemistry (left panel; scale bar=5 μm) and quantification of PdG (right panel) in PDGF-treated VSMCs with or without SLC25A33 -targeting siRNA. (E) Representative immunofluorescence images of double-stranded DNA (dsDNA) and mitochondria (left panel; scale bar=10 μm) and quantification of cytosolic dsDNA foci (right panel) in PDGF-treated VSMCs with or without SLC25A33 -targeting siRNA. si SLC25A33 is referred to as siA33. Data in the bar graphs represent the mean±standard error of the mean of three independent measurements. Ctrl, control; NS, not significant; DAPI, 4’,6-diamidino-2-phenylindole. a P <0.05, b P <0.01, c P <0.001.
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Solute carrier family 25 member 33 (SLC25A33) regulates mitochondrial ROS (mtROS) and mitochondrial DNA (mtDNA) production in response to platelet-derived growth factor (PDGF), thereby promoting mtDNA release into the cytosol. (A) Representative immunofluorescence images of MitoSOX Red (left panel; scale bar=10 μm) and quantification of fluorescence intensity (right panel) in PDGF-treated vascular smooth muscle cells (VSMCs) with or without SLC25A33 -targeting small interfering RNA (siRNA). (B) Effect of SLC25A33 knockdown on mitochondrial and cytosolic uridine 5’-triphosphate (UTP) levels in PDGF-treated VSMCs. (C) Effect of SLC25A33 knockdown on total mtDNA levels in PDGF-treated VSMCs. (D) Representative immunofluorescence images <t>acquired</t> <t>using</t> <t>6-O-propynyl-dG</t> (PdG) and click chemistry (left panel; scale bar=5 μm) and quantification of PdG (right panel) in PDGF-treated VSMCs with or without SLC25A33 -targeting siRNA. (E) Representative immunofluorescence images of double-stranded DNA (dsDNA) and mitochondria (left panel; scale bar=10 μm) and quantification of cytosolic dsDNA foci (right panel) in PDGF-treated VSMCs with or without SLC25A33 -targeting siRNA. si SLC25A33 is referred to as siA33. Data in the bar graphs represent the mean±standard error of the mean of three independent measurements. Ctrl, control; NS, not significant; DAPI, 4’,6-diamidino-2-phenylindole. a P <0.05, b P <0.01, c P <0.001.
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Solute carrier family 25 member 33 (SLC25A33) regulates mitochondrial ROS (mtROS) and mitochondrial DNA (mtDNA) production in response to platelet-derived growth factor (PDGF), thereby promoting mtDNA release into the cytosol. (A) Representative immunofluorescence images of MitoSOX Red (left panel; scale bar=10 μm) and quantification of fluorescence intensity (right panel) in PDGF-treated vascular smooth muscle cells (VSMCs) with or without SLC25A33 -targeting small interfering RNA (siRNA). (B) Effect of SLC25A33 knockdown on mitochondrial and cytosolic uridine 5’-triphosphate (UTP) levels in PDGF-treated VSMCs. (C) Effect of SLC25A33 knockdown on total mtDNA levels in PDGF-treated VSMCs. (D) Representative immunofluorescence images <t>acquired</t> <t>using</t> <t>6-O-propynyl-dG</t> (PdG) and click chemistry (left panel; scale bar=5 μm) and quantification of PdG (right panel) in PDGF-treated VSMCs with or without SLC25A33 -targeting siRNA. (E) Representative immunofluorescence images of double-stranded DNA (dsDNA) and mitochondria (left panel; scale bar=10 μm) and quantification of cytosolic dsDNA foci (right panel) in PDGF-treated VSMCs with or without SLC25A33 -targeting siRNA. si SLC25A33 is referred to as siA33. Data in the bar graphs represent the mean±standard error of the mean of three independent measurements. Ctrl, control; NS, not significant; DAPI, 4’,6-diamidino-2-phenylindole. a P <0.05, b P <0.01, c P <0.001.
Clk, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
clk - by Bioz Stars, 2026-10
94/100 stars
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Image Search Results


Solute carrier family 25 member 33 (SLC25A33) regulates mitochondrial ROS (mtROS) and mitochondrial DNA (mtDNA) production in response to platelet-derived growth factor (PDGF), thereby promoting mtDNA release into the cytosol. (A) Representative immunofluorescence images of MitoSOX Red (left panel; scale bar=10 μm) and quantification of fluorescence intensity (right panel) in PDGF-treated vascular smooth muscle cells (VSMCs) with or without SLC25A33 -targeting small interfering RNA (siRNA). (B) Effect of SLC25A33 knockdown on mitochondrial and cytosolic uridine 5’-triphosphate (UTP) levels in PDGF-treated VSMCs. (C) Effect of SLC25A33 knockdown on total mtDNA levels in PDGF-treated VSMCs. (D) Representative immunofluorescence images acquired using 6-O-propynyl-dG (PdG) and click chemistry (left panel; scale bar=5 μm) and quantification of PdG (right panel) in PDGF-treated VSMCs with or without SLC25A33 -targeting siRNA. (E) Representative immunofluorescence images of double-stranded DNA (dsDNA) and mitochondria (left panel; scale bar=10 μm) and quantification of cytosolic dsDNA foci (right panel) in PDGF-treated VSMCs with or without SLC25A33 -targeting siRNA. si SLC25A33 is referred to as siA33. Data in the bar graphs represent the mean±standard error of the mean of three independent measurements. Ctrl, control; NS, not significant; DAPI, 4’,6-diamidino-2-phenylindole. a P <0.05, b P <0.01, c P <0.001.

Journal: Diabetes & Metabolism Journal

Article Title: Targeting SLC25A33 Suppresses Vascular Smooth Muscle Cell Proliferation and Migration by Reducing Cytosolic mtDNA Levels: Implications for Occlusive Vascular Diseases

doi: 10.4093/dmj.2024.0632

Figure Lengend Snippet: Solute carrier family 25 member 33 (SLC25A33) regulates mitochondrial ROS (mtROS) and mitochondrial DNA (mtDNA) production in response to platelet-derived growth factor (PDGF), thereby promoting mtDNA release into the cytosol. (A) Representative immunofluorescence images of MitoSOX Red (left panel; scale bar=10 μm) and quantification of fluorescence intensity (right panel) in PDGF-treated vascular smooth muscle cells (VSMCs) with or without SLC25A33 -targeting small interfering RNA (siRNA). (B) Effect of SLC25A33 knockdown on mitochondrial and cytosolic uridine 5’-triphosphate (UTP) levels in PDGF-treated VSMCs. (C) Effect of SLC25A33 knockdown on total mtDNA levels in PDGF-treated VSMCs. (D) Representative immunofluorescence images acquired using 6-O-propynyl-dG (PdG) and click chemistry (left panel; scale bar=5 μm) and quantification of PdG (right panel) in PDGF-treated VSMCs with or without SLC25A33 -targeting siRNA. (E) Representative immunofluorescence images of double-stranded DNA (dsDNA) and mitochondria (left panel; scale bar=10 μm) and quantification of cytosolic dsDNA foci (right panel) in PDGF-treated VSMCs with or without SLC25A33 -targeting siRNA. si SLC25A33 is referred to as siA33. Data in the bar graphs represent the mean±standard error of the mean of three independent measurements. Ctrl, control; NS, not significant; DAPI, 4’,6-diamidino-2-phenylindole. a P <0.05, b P <0.01, c P <0.001.

Article Snippet: To evaluate mtDNA replication in VSMCs, the cells were incubated with or without PDGF in the presence of 6-O-propynyl-dG (PdG; Jena Bioscience, Jena, Germany) and analyzed using a CuAAC Cell Reaction Buffer Kit (BTTAA-based) (Jena Bioscience).

Techniques: Derivative Assay, Immunofluorescence, Fluorescence, Small Interfering RNA, Knockdown, Control